Beatrice Vallone, Ph.D.

Beatrice Vallone obtained her degree in Biology and ph.D. in Biochemistry at the Sapienza University of Rome, from 2007 she is Full Professor of Biochemistry in the Department of Biochemical Sciences “A.Rossi Fanelli” of Sapienza University. In 1991 she became Assistant Professor in Biochemistry and Associate Professor of Biochemistry in 2000, always at the Sapienza University of Rome.

She has spent several periods abroad, for her PhD, post-doc and as a visiting professor, working at the following institutions: LMB-MRC (Cambridge-UK), Geneva University (CH), York University (UK), Cambridge University (UK), Columbia University as Shaefer Research Scholar (NY, USA) and as Alexander Bodini Resarch Fellow Italian Academy for Advanced Studies in America at Columbia University (NY, USA) and at the Nanyang Technological University (Singapore).

She is author of more than 90 papers published in peer-reviewed international journals (H-index 31) and one patent; she has been invited to present her work in over 30 national and international meetings. Her research is funded by national (CNR, Sapienza Univ., Institute Pasteur-Fondazione Cenci Bolognetti, AIRC) and international agencies (EC X-Probe and Prometeus). She has ongoing collaborations with European and international groups.

She founded the CNR-Sapienza Biocrystal Facility in 2011 and coordinated it until 2017, presently part of the Istruct Italy Network.

From 2017 she is member of the Scientific Board of the H2CU Interuniversity Center of which she was elected Director in 2019.

From 2017 she is Chair of the International Committee for project evaluation at the Italian National Elettra Synchrotron.

From 2017 she is member of the Biological Macromolecules Commission dell’International Union for Crystallography (IUCr)

From 2010 until 2014 she was elected as representative for Structural Biology in the ESRF User Organization Committee.

From 2020 she coordinates the Italian beam time allocation group at the ESRF for Cryo-electron microscopy.

Dr Carmelinda Savino CNR

Carmelinda Savino, Ph.D.

Carmelinda is researcher at the Institute of Molecolar Biology and Pathology, National Reaserch Council (IBPM-CNR).

She gradued in Chemistry and obtained the Ph.D, in Chemistry at the “Federico II” University of Naples.

She has spent several periods abroad working at the following institutions: Department of Chemistry, University of York (UK), Architecture et fonction des Macromolécules Biologiques”, CNRS, Marseille (F),Department of Physiology and Cellular Biophysics, Columbia University Medical Center (CUMC), New York (USA).

The main focus of her research has been on the crystallographic analysis of proteins, in order to gain insight of biochemical and biological phenomena. In order to understand the mechanism of action of protein/enzyme, the structural information is integrated with functional characterization and protein engineering. Some of the lines are centered on biotechnological and biomedical applications. As a result of this research activity, she has published scientific articles on peer-reviewed journals in collaboration with italian and foreign researchers or books and one patent.

Linda Celeste Montemiglio, Ph.D.

Linda Celeste is a staff researcher at the Institute of Biology and Cellular Pathology, National Research Council (CNR), Rome, Italy.

She graduated in Chemistry and obtained a Ph.D. in Biochemistry at the University of Rome, Sapienza (Rome, Italy). During her post-doctorate, she worked in the lab of Prof. Filippo Mancia at Columbia University (CUMC) and in the lab of Prof. Amedee des Georges at the City University of New York (CUNY) in New York (USA).

She is a structural biologist with experience in x-ray crystallography, single particle cryo-EM, and other biophysical techniques for functional studies.

Her research is focused on the structural and functional characterization of biotechnologically relevant enzymes involved in antibiotic biosynthesis, on the study of macromolecular complexes involved in cancer development, and on engineering and characterizing proteins to produce selective drug delivery nano-vehicles for biomedical applications.

Cécile Exertier, Ph.D.

Cécile obtained a B.S. in Chemistry and Biology and a M.S. in Biochemistry and Structural Biology at the University Joseph Fourier (Grenoble, France).

In December 2017, she was awarded a Ph.D. in Biochemistry from the University of Rome “La Sapienza” which was funded by the Horizon2020 “Marie Sklodowska-Curie” ITN-ETN program (X-Probe network → http://x-probe.org/projects/time-resolved-diffraction-waxs-studies-of-heme-proteins/?portfolioID=76).

During her doctorate, she aimed at investigating heme proteins structural dynamics using static and time-resolved X-ray methods, which led her to carry out collaborative experiments at the SLAC XFEL (California) and at ESRF (France).

Her current post-doc research project focuses on the structural characterization of a human ternary complex involved in hemoglobin clearance involving hemoglobin, haptoglobin and CD163 macrophage receptor by cryo-EM.

Ida Freda

Ida obtained a B.S. in Biotechnology at Tor Vergata University and a M.S. in Medical Biotechnology at Sapienza University.

As an undergraduate intern, she focused on the structure-function relationship of heme proteins by means of protein engineering, X-ray crystallography and spectroscopic techniques.

In 2018 she became a fellow at the PhD course in Biochemistry at Dept. of Biochemical Sciences of Sapienza University, where she is currently working on the structural characterization by single particle cryo-electron microscopy of a ternary complex that mediate hemoglobin scavenging and clearance.

Another research project focuses on the structural and functional characterization of a membrane enzyme family involved in steroid metabolism, carried out in collaboration with Columbia University of New York.

Elena Gugole

Elena graduated in Biology (March 2017 – Bachelor’s Degree) and in Genetics and Molecular Biology (July 2019 – Master’s Degree) at Sapienza University of Rome.

She carried her Master’s thesis work under the supervision of Prof. Beatrice Vallone and Dr. Carmelinda Savino on the functional and structural characterization of a bacterial Cytochrome P450 involved in the biosynthetic pathway of an antibiotic.

In November 2019 she beame a fellow of the PhD School in Life Sciences at Sapienza University of Rome. Her doctoral work is focused on the structural and functional characterization of proteins involved in the biosynthesis of antibiotics and fungus-derived proteins with applications in green chemistry and pharmaceutics, thanks to a collaboration started during her master thesis with INRA-Aix-Marseille University (Marseille, France). She visited the INRA Laboratories for collaborative experimental activities and training.

Andrea Mangullo, ENGR

Andrea obtained a B.S. in Chemical Engineering, with a minor in Material Science, and a M.S. in Nanotechnology Engineering, with a minor in Biotechnology, at the University of Rome “La Sapienza” (Rome, Italy).

In 2018, he worked as scientific researcher at Cees Dekker Lab (https://ceesdekkerlab.nl), Kavli Institute of Nanoscience of Delft University of Technology (Delft, The Netherlands). During the period he spent at the TU Delft, Andrea was primarily involved in the fabrication by IBL, surface engineering and TEM imaging of solid-state nanopores combined with intrinsically disordered proteins of the Saccharomyces cerevisiae Nuclear Pore Complex.

In November 2019, he was awarded a scholarship from the “Istituto Pasteur – Fondazione Cenci Bolognetti” for his research project, which focuses on designing and fabricating newly-conceived Graphene-based support films for the structural characterization of protein complexes by Cryo-EM. The project is supported by the “Open Infrastructure for Advanced TOmography and Microscopies” (ATOM) framework (http://www.atomcenter.org), in collaboration with the Department of Basic and Applied Sciences for Engineering, University of Rome “La Sapienza”, and the Institute for Microelectronics and Microsystems, National Research Council of Bologna, Italy.

Lucia D’Auria

Lucia graduated in Chemistry (July 2018 – Bachelor’s Degree) at the University of Rome “La Sapienza”, Italy.

She studies Chemistry of Biological Systems (Master’s Degree) and her current master thesis in structural biology is focused on the functional and structural characterization of a bacterial cytochrome P450 using X-ray crystallography, molecular biology and biophysical approaches.